Check out the Various Biochemical Tests For Streptococcus pneumoniae (S. pneumoniae)
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There are so many biochemical reactions for the well known causative agent of Pneumonia and Otitis media infection i.e. the Streptococcus pneumoniae but a few reactions are most commonly used and are medically important for distinguishing pathogenic strains of Streptococcus pneumoniae from other non- pathogenic strains as well as from other species of Streptococcus which are as follows:
[wp-svg-icons icon=”point-right” wrap=”i”] Catalase Negative
[wp-svg-icons icon=”point-right” wrap=”i”] Oxidase Negative
[wp-svg-icons icon=”point-right” wrap=”i”] Alfa- hemolysis on Blood agar
[wp-svg-icons icon=”point-right” wrap=”i”] Soluble in 10% Bile (differentiate S. pneumoniae from S. pyogenes)
[wp-svg-icons icon=”point-right” wrap=”i”] Growth on CVNG (Crystal violet – Nalidixic acid – Gentamycin Agar) medium.
[wp-svg-icons icon=”point-right” wrap=”i”] Fermentation of Inulin (differentiate S. pneumoniae from other Streptococci)
[wp-svg-icons icon=”point-right” wrap=”i”] Sensitive to the Optochin antibiotic (differentiate S. pyogenes from other Hemolytic Streptococci)
Besides that of various biochemical reactions and Sugar fermentation, certain enzymatic reactions are also medically important to distinguish Streptococcus pneumoniae from other Streptococci.
Below is the list of these Enzymatic Reactions and various other biochemical tests for Streptococcus pneumoniae which have great importance in research and for knowledge but are not routinely employed:
[table id=99 /]
Streptococcus pneumoniae and other species of Streptococci ferment various sugars producing acid without gas.
But these sugar fermentation tests are of no diagnostic value in routine laboratory tests except Inulin fermentation test which is of great importance in differentiating Streptococcuspneumoniae, which is Inulin fermentation Positive, from other Pathogenic & non-pathogenic Streptococci especially from Streptococcus pyogenes.
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The other sugar fermentation tests which are described below are of great importance in research work.
[table id=100 /]