Hey, Good to see you here 😀 …… In this Article, we’re gonna discuss in detail about various biochemical tests for the Staphylococcus aureus….. If you have any queries, don’t forget to mention in Comments…Thanks
There are so many biochemical tests for Staphylococcus aureus, the well known causative agent of localized suppurative lesions.
However, only a few reactions are most commonly used and are medically important for distinguishing pathogenic staphylococcus i.e. S. aureus from other non- pathogenic Staphylococci which are as follows:
Beta hemolysis on Blood agar
Golden yellow pigment production
Deoxyribonuclease (DNase) production
Below is the list of various other biochemical tests for Staphylococcus aureus which have great importance in research:
Catalase +VE (POSITIVE)
Gelatin Hydrolysis +VE (POSITIVE)
H2S production -VE (NEGATIVE)
Gas production -VE (NEGATIVE)
Indole -VE (NEGATIVE)
MR (Methyl Red) +VE (POSITIVE)
VP (Voges Proskauer) +VE (POSITIVE)
Citrate +VE (POSITIVE)
PYR -VE (NEGATIVE)
Urease +VE (POSITIVE)
DNase +VE (POSITIVE)
Oxidase -VE (NEGATIVE)
Phosphatase +VE (POSITIVE)
Nitrate Reduction +VE (POSITIVE)
Hyalurodinase +VE (POSITIVE)
Lipase +VE (POSITIVE)
Staphylococcus aureus and other species of staphylococci ferment various sugars producing acid without gas.
However, these sugar fermentation tests are of no diagnostic value in routine laboratory tests except Mannitol fermentation which is of great importance in differentiating Staphylococcus aureus which is mannitol positive from other non-pathogenic Staphylococci, especially from Staphylococcus epidermidis.
The various other sugar fermentation tests are of great importance in research work and for knowledge:
Glucose +VE (POSITIVE)
Fructose +VE (POSITIVE)
Lactose +VE (POSITIVE)
Galactose +VE (POSITIVE)
Maltose +VE (POSITIVE)
Arabinose -VE (NEGATIVE)
Mannitol +VE (POSITIVE)
Mannose +VE (POSITIVE)
Raffinose -VE (NEGATIVE)
Ribose +VE (POSITIVE)
Sucrose +VE (POSITIVE)
Xylose -VE (NEGATIVE)
That’s all about Biochemical Tests for Staphylococcus aureus.
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