Home » KLEBSIELLA PNEUMONIAE » Check out the Morphology and Culture Characteristics Of Klebsiella pneumoniae (K. pneumoniae)
KLEBSIELLA PNEUMONIAE MORPHOLOGY OF BACTERIA

Check out the Morphology and Culture Characteristics Of Klebsiella pneumoniae (K. pneumoniae)

Morphology of Klebsiella pneumoniae - laboratory hub - Culture characteristics of Klebsiella pneumoniae - klebsiella morphology - shape - size - arrangement

Hey, Good to see you here 😀 …… In this Article, We’re gonna discuss in Detail the Morphology & Culture characteristics of Klebsiella pneumoniae….. If you have any queries, don’t forget to mention in Comments….. Thanks

Morphology of Klebsiella pneumoniae

Klebsiella pneumoniae is a short, plump, straight rod shape (bacillus) bacterium.



The size of Klebsiella pneumoniae is about 1–2 µm × 0.5–0.8 µm (micrometer).

K. pneumoniae is arranged singly, in pairs, or in short chains and sometimes in clusters.

Morphology of klebsiella pneumoniae - laboratory hub - capsuated gram negative rod
Morphology Of Klebsiella pneumoniae

Klebsiella pneumoniae is a non-motile bacterium.

K. pneumoniae is a non-flagellated bacterium.

The Klebsiella pneumoniae is a non–sporing bacterium.

Capsules are present in Klebsiella pneumoniae which can easily be demonstrated using India ink preparation, appear as a clear halo in a dark background.

Klebsiella pneumoniae is a Gram -ve (Negative) bacterium.

Culture Requirements of Klebsiella pneumoniae (K. pneumoniae)

Special requirementsKlebsiella pneumoniae have no complex nutritional requirements and readily grow in an ordinary media like Nutrient Agar medium (NAM). Commonly the NAM & MacConkey Agar medium is used for the cultivation of Klebsiella pneumoniae in Laboratory.




Optimum temperatureKlebsiella pneumoniae ranges from 15–40°C but the optimum temperature for most of the strains is 37°C and usually cultivated at this temperature in laboratories.

Optimum pHK. pneumoniae can survive alkaline pH but not at acidic pH and the maximum growth of the organism is observed at 7.0 i.e. neutral pH. Also, the pH requirement varies as per the strain of Klebsiella pneumoniae.

Oxygen requirementsKlebsiella pneumoniae (K. pneumoniae) is an aerobic bacterium i.e. grow best in the presence of oxygen and it is also a Facultative anaerobic organism i.e. can grow in the low oxygen environment.

⇒ There are various culture media used for the cultivation of Klebsiella pneumoniae in the laboratory and most commonly the Nutrient Agar medium and MacConkey Agar medium is used, the other media are as follows –

  • Columbia Horse Blood Agar medium
  • Sheep Blood Agar medium
  • Trypticase Soy Agar medium
  • Eosin Methylene Blue Agar (EMB Agar) Medium.
  • The liquid medium (Nutrient Broth medium, TSB medium, etc.).

 The Eosin Methylene Blue Agar (EMB Agar) medium which is the Selective medium for Klebsiella pneumoniae contains Eosin dye and Methylene Blue dye which inhibits the growth of many gram-positive bacteria, inhibits the growth of Shigella and Salmonella species and supports the growth of Klebsiella pneumoniae.




Culture Characteristics of Klebsiella pneumoniae (K. pneumoniae)

Cultural CharacteristicsNutrient Agar Medium (NAM)MacConkey Agar mediumBlood Agar MediumEMB Agar medium
ShapeCircularCircularCircularCircular
Size2-3 mm2-3 mm2-3 mm2-3 mm
ElevationDome-shapedConvexDome-shapedConvex
SurfaceMucoidMucoidMucoidMucoid
ColorGreyish whitePink – RedGreyish whitePink – Purple
StructureTranslucent–OpaqueOpaqueTranslucent–OpaqueTranslucent–Opaque
Hemolysis----------γ-Hemolysis (Non-hemolytic)-----

In Liquid culture media like Trypticase soy broth or Nutrient broth, the growth of the bacterium occurs as a turbidity in the broth medium which is further analyzed for the morphology (under the microscope), gram reaction, biochemical tests, and Klebsiella pneumoniae specific tests.

In Blood Agar medium, the Klebsiella pneumoniae colonies are non-hemolytic i.e. shows Gamma Hemolysis (γ-hemolysis).

klebsiella pneumoniae on blood agar medium - laboratory hub - klebsiella on lood agar
Culture Of Klebsiella Pneumoniae On Blood Agar Medium

In MacConkey Agar medium, the colonies of Klebsiella pneumoniae are pink colored due to the lactose fermentation which is of great importance in differentiating K. pneumoniae from other Bacteria present in the specimen, especially from Gram-positive bacteria and Salmonella species which are non–lactose fermentors and gives colorless colonies on MacConkey agar medium.

klebsiella pneumoniae on macconkey agar medium - laboratory hub - klebsiella on macconkey agar medium - k. pneumoniae on macconkey agar medium
Culture Of Klebsiella Pneumoniae On Macconkey Agar

In Eosin Methylene Blue Agar (EMB) medium, the colonies of Klebsiella pneumoniae are Pink to purple in color without green metallic sheen which is of great importance in differentiating K. pneumoniae from other bacteria normally found in Specimen and especially from E. coli which grows with Green metallic sheen.

Klebsiella pneumoniae on EMB medium - laboratory hub - klebsiella pneumoniae on eosin methylene blue medium
Culture Of Klebsiella Pneumoniae On EMB Medium

That’s all about the Morphology and Culture Characteristics of Klebsiella pneumoniae….



Sending
User Review
0 (0 votes)

Related Articles

Check out the Morphology And Culture Characteristics Of Yersinia pestis (Y. pestis)

SAHIL BATRA

Check out the various Biochemical Tests For Klebsiella pneumoniae (K. pneumoniae)

SAHIL BATRA

Check out the Morphology And Culture Characteristics Of Pseudomonas aeruginosa (P. aerugunosa)

SAHIL BATRA

Check out the Morphology and Culture Characteristics of Shigella dysenteriae (Sh. dysenteriae)

SAHIL BATRA

Leave a Comment

* By using this form you agree with the storage and handling of your data by this website.

This website uses cookies to improve your experience. We'll assume you're ok with this. Accept Read More